All trees generated in this study can be viewed in and exported from iTol connexion: calvignacs, project: Ancient recombination events between human herpes simplex viruses. Herpes simplex viruses 1 and 2 HSV-1 and HSV-2 are seen as close relatives but also unambiguously considered as evolutionary independent units. Here, we sequenced the genomes of 18 HSV-2 isolates characterized by divergent UL30 gene sequences to further elucidate the evolutionary history of this virus. Surprisingly, genome-wide recombination analyses showed that all HSV-2 genomes sequenced to date contain HSV-1 fragments. Using phylogenomic analyses, we could also show that two main HSV-2 lineages exist.
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